Part:BBa_K1634008

pmyo2-dsRed ( C.elegans )

Pmyo2(BBa_K1634002) is a promoter which can drive the expression in the muscle of C.elegans especially the muscle in the head and pharynx. DsRed(BBa_K1634006) is a commonly used fluorescent protein,a reporter. If we want\ to express some special protein such as channelrhodopsins in those muscle, we need to inject the mixtures containing the plasmid we constructed into the sexual gland of the C.elegans. Then we can get the transgenic descendants we want. However, this dosen't mean that we can get some successful transgenic descendants after every microinjection. So we need to pick up the several successful ones from thousands of descendants. But sometimes the specific protein we express has not contained a reporter, and even though the ChR2 we express contains a YFP, the light is not strong enough for us to find the successful one from thousands of descendants. To solve this problem, we constructed this part, Pmyo2-dsRed ,which is called "the comarker"by us. We make mixture of Pmyo2-dsRed and Pmyo2-channelrhodopsin, then inject the mixture through microinjection. The possible red fluorescent in the descendants has 4 main functions. Firstly, it ensures that the microinjection process is successful. Secondly, it ensures that those plasmids has successfully passed to the descendants. Thirdly, since it has the same promoter as our target protein, we can assume that our target protein has also successfully expressed in the same position, and we can have a double-check if our protein has a reporter. Finally, it can also help us to learn the expression pattern and quantify the expression level roughly by observing the fluorescence intensity of dsRed.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 288
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1178
  Illegal SapI.rc site found at 198